简介：TheNetAcetmethodhasbeendevelopedtomakepredictionsofN-terminalacetylationsites,butmoreinformationofthedatasetcouldbeutilizedtoimprovetheperformanceofthemodel.Byemployinganewwaytoextractpatternsfromsequencesandusingasamplebalancingmechanism,weobtainedacorrelationcoefficientof0.85,andasensitivityof93%onanindependentmammaliandataset.Awebserverutilizingthismethodhasbeenconstructedandisavailableathttp://166.111.24.5/acetylation.html.

简介：Kr眉p像像素的因素8(KLF8)抄写因素在房间周期前进起一个关键作用，oncogenic转变，对间充质的转变和侵略上皮。然而，它的原子本地化信号(NLS)没被识别。有另外的KLFmonopartiteNLS(mNLS)和C2H2锌手指(ZF)的KLF8份额，哪个被显示了是为一些另外的KLF的NLS。在这份报告，用指导PCR的mutagenesis和immunofluorescent显微镜学，我们显示出mNLSs，任何单个ZF的删除，或变化的那混乱Zn2+有约束力或联系DNA主题没影响KLF8的原子本地化。删除>然而，从C终点的1.5ZF引起了KLF8的细胞质的累积。令人惊讶地，氨基酸(aa)的删除151-200区域几乎从原子核消除了KLF8。有PKC禁止者的S165A，K171E或K171R变化，或处理导致了部分细胞质的累积。Co-immunoprecipitation证明KLF8与importin-交往了，这个相互作用要求了ZF主题。aa1-150或201-261区域的删除独自没改变原子本地化。BrdU加入和cyclinD1倡导者酶试金作为野类型的KLF8证明在原子本地化的KLF8异种有缺陷者不能支持DNA合成或cyclinD1倡导者激活。一起拿，这些结果建议KLF8有二NLS，一包围S165和K171并且另外的是二双人脚踏车ZF，它为KLF8原子本地化和它的细胞的功能的规定是批评的。

简介：Humanrhodopsinkinase(RK)andacarboxylterminus-truncatedmutantRKlackingthelast59aminoacids(RKC)wereexpressedinhumanembryonickidney293cellstoinvestigatetheroleofthecarboxylterminusofRKinrecognitionandphosphorylationofrhodopsin.RKC,likethewild-typeRK,wasdetectedinbothplasmamembranesandcytosolicfractions.TheCterminaltruncatedrhodopsinkinasewasunabletophosphorylatephoto-activatedrhodopsin,butpossesseskinaseactivitysimilartothewild-typeRKinphosphorylationofsmallpeptidesubstrate.ItsuggeststhatthetruncationdidnotdisturbthegrossstructuresofRKcatalyticdomain.OurresultsalsoshowthatRKCfailedtotranslocatetophoto-activatedrodoutsegments.Takentogether,ourstudydemonstratethecarboxylterminusofRKisrequiredforphosphorylationofphoto-activatedrhodopsinandstronglyindicatethatcarboxyl-terminusofRKmaybeinvolvedininteractionwithphoto-activatedrhodopsin.

简介：InordertodevelopclinicaldiagnostictoolsforrapiddetectionofSARS-CoV(severeacuterespiratorysyndrome-associatedcoronavirus)andtoidentifycandidateproteinsforvaccinedevelopment,theC-terminalportionofthenucleocapsid(NC)genewasamplifiedusingRT-PCRfromtheSARS-CoVgenome,clonedintoayeastexpressionvector(pEGH),andexpressedasaglutathioneS-transferase(GST)andHisx6double-taggedfusionproteinunderthecontrolofaninduciblepromoter.WesternanalysisonthepurifiedproteinconfirmedtheexpressionandpurificationoftheNCfusionproteinsfromyeast.Todetermineitsantigenicity,thefusionproteinwaschallengedwithserumsamplesfromSARSpatientsandnormalcontrols.TheNCfusionproteindemonstratedhighantigenicitywithhighspecificity,andtherefore,itshouldhavegreatpotentialindesigningclinicaldiagnostictoolsandprovideusefulinformationforvaccinedevelopment.

简介：P28，a28kDproteinfromtoad(Bufobufogargarizans)oocytes,wasidentifiedbyusingP13^suc1-agaroseaffinitychromatography.Sequencehomologyanalysisofthefull-lengthcDNAofP28(GeneBankaccessionnumber:AF314091)indicatedthatitencodesaproteincontaining224amino-acidswithabout55%iden-titiesandmorethan70%positivestoencodesaproteincontaining224amino-acidswithabout55%iden-titiesandmorethan70%positivestohuman,ratormouseUCH-L1,andcontainshomologicalfunctionaldomainsofUCHfamily.Anti-p28monoclonalantibody,oninjectingintotheoocytes,couldinhibittheprogesterone-inducedresumptionofmeioticdivisioninadose-dependentmanner.TherecombinantproteinP28showedsimilarSDS/PAGEbehaviorstothenativeone,andpromotedubiquitinethylesterhydrolysis,aclassicalcatalyticreactionforubiquitincarboxylterminalhydrolases(UCHs).Theresultsinthispaperrevealthatanovelprotein,p28,existsinthetoadoocytes,isaUCHLlhomolog,wasengagedintheprocessofprogesterone-inducedoocytematurationpossiblythroughaninvolvementinproteinturnoveranddegradation.

简介：预言潜水艇的能力从它的顺序的蛋白质的细胞的本地化是很重要的，它关于蛋白质的功能提供信息。我们在场一个计算工具，PredSL，它利用神经网络，Markov链，隐藏的Markov建模的侧面，和得分为潜水艇的预言的矩阵在从N终端氨基酸顺序的真核细胞的房间的蛋白质的细胞的本地化。它试图分类蛋白质进五个组：叶绿体，thylakoid，线粒体，能分泌的小径，和“其它”。当在一个五倍的交叉验证过程测试了时，PredSL分别地为植物和非植物数据集表明86.7%和87.1%全面精确性。与TargetP，迄今为止是最广泛地使用的方法，和LumenP相比，PredSL的结果在大多数情况中是可比较的。当在Saccharomycescerevisiae染色体的试验性地验证的蛋白质上测试了时，PredSL可比较地表现如果比为一样的任务的任何可得到的算法不好。而且，PredSL是为这些的预言有能力的唯一的方法代替作为通过URL:http://bioinformatics.biol.uoa.gr/PredSL/的独立应用可得到的细胞的本地化。

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简介：Thenorepinephrinetransporter(NET)isamemberoftheNa^+/Cl^-dependentneurotransmittertransporterfamilyandconstitutesthetargetofseveralclinicallyimportantantidepressants.TodelineatethecriticalaminoacidresiduesandthefunctionofC-terminalinregulatingtransportactivityofNET,hereweconstructedtwositemutants(V70F,F72V;V70I,F72V)andoneC-terminaltruncatedmutant(Δ611-617).ThewildtypeandmutantsofNETwereexpressedinXenopusoocytesbyinjectionoftheircRNA.Wefoundthatallofthesemutantslosttheirtransportactivity.TheseresultsindicatethattheaminoacidresiduesofV70andF72,andthelastsevenaminoacidsofC-terminalareessentialtothetransportactivityofNET.

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