简介:AIM:Toinvestigatetheefficacyofnon-buckledvitrectomywithclassicalendotamponadeagentsinthetreatmentofprimaryretinaldetachment(RD)complicatedbyinferiorbreaksandproliferativevitreoretinophathy(PVR).METHODS:Aretrospective,consecutiveandcaseseriesstudyof40patientswithinferiorbreakRDandPVR≥C1wasconducted.Allpatientsunderwentastandard3-port20-gaugeparsplanavitrectomy(PPV)withgasorsiliconeoiltamponadewithoutsupplementaryscleralbuckling.Thevitreousandallproliferativemembranewerecompletelyremoved,andretinectomywasperformedwhennecessary.Themeanfollow-upwas12.5months.Theprimaryandfinalanatomicsuccessrate,visualacuityandcomplicationswererecordedandanalyzed.RESULTS:Primaryanatomicsuccessratewasachievedin35of40eyes(87.5%)andthefinalanatomicsuccessratewas100%.ThemostcommoncauseofredetachmentwasrecurrentPVR.Thebest-correctedvisualacuity(BCVA)atfinalfollow-upwasimprovedin34eyes(85%),remainedstablein1eye(2.5%),andworsenedin5eyes(12.5%).Themeanvisualacuityatfinalfollow-upwasimprovedsignificantly(P=0.000).CONCLUSION:ThisretrospectivestudyprovidesevidencethatvitrectomywithoutscleralbucklingseemedtobeaneffectivetreatmentforinferiorbreakRDwithPVR.Withcompleteremovalofvitreousandproliferativemembranesandtimingofretinectomy,theinferiorbreakswhichcomplicatedwithPVRcouldbeclosedsuccessfullywithoutadditionalscleralbuckling.
简介:目的:应用Pentacam眼前节测量分析系统对高度近视眼行飞秒激光LASIK和小切口基质透镜取出术后的角膜后表面的变化进行观察比较。方法:选取2015-05/12于我院行激光角膜屈光手术的高度近视患者(-6.00D≤等效球镜度数≤-10.00D)67例132眼,根据患者接受手术方式的不同分成飞秒激光制瓣联合准分子激光原位角膜磨镶术(简称FS-LASIK组)66眼和行小切口基质透镜取出术(简称SMILE组)66眼,术中SMILE组角膜帽厚度和FS-LASIK组术中角膜瓣厚度都设置为110μm。分别于术前、术后3、6mo利用Pentacam测量患者角膜后表面高度,观察对比其术前术后的角膜后表面高度的变化量。结果:术后6mo,FS-LASIK组角膜后表面高度为6.47±1.65mm,显著高于术前5.20±1.32mm。SMILE组角膜后表面高度为6.40±1.33mm,显著高于术前5.18±1.25mm,差异均具有统计学意义(P〈0.05);术后6mo,FS-LASIK组角膜表面高度前凸量(1.29±1.28mm)与SMILE组(1.22±0.89mm)相当,差异无统计学意义(P〉0.05)。结论:FS-LASIK和SMILE矫正高度近视术后均出现一定程度的角膜后表面前突,后表面高度值增加;FS-LASIK相较于SMILE,术后早期角膜后表面变化量略大,但无明显的临床差别,SMILE术后角膜后表面形态稳定性相对略好。
简介:AIM:Todescribethedesignandpreliminaryresultsofthehospitalbasedepidemiologicalstudyfordiabeticretinopathy(HBESDR),anongoingepidemiologicalstudytoestimatetheprevalenceofdiabeticretinopathy(DR)andtoelucidatetheclinical,anthropometric,biochemicalandanyotherriskfactorsassociatedwithdiabeticretinopathy.METHODS:Totally2000diabeteswillberecruitedfromtheDiabeteseyeclinicintheFirstAffiliatedHospitalofChinaMedicalUniversity.AllsubjectsunderwentbloodsugarestimationandOralGlucoseToleranceTesttodiagnosediabetes.Alldiabeteswouldundergocompletequestionnaire,acomprehensiveeyeexamination.Bloodandurinewouldbecollectedforbiochemicalinvestigations.AllfundusphotographsforanyDRwillbegraded.Participantswhoneedtreatmentwillbesenttotheophthalmicclinicandfollow-upintervalprogramforallsubjectswillbesuggested.Acomputerizeddatabaseiscreatedfortherecords.RESULTS:Todate,1174diabeteshavebeenrecruited,therewere350(29.81%)DRinalldiabetes,mostofthemwerewithmildnon-proliferativediabeticretinopathy(NPDR)(139,39.71%);71(20.29%)moderateNPDR,66(18.86%)severeNPDR,74(21.14%)proliferativediabeticretinopathy(PDR).Females,longerdurationofdiabetes,familyhistoryofdiabetesandhypertensionhadastatisticallysignificantincreaseinriskofanyDR.CONCLUSION:ThestudyisexpectedtoprovideanestimateoftheoverallprevalenceofDRandtheprevalencewithdifferentdurationofdiabetesandalsoabetterunderstandingoftheriskfactorsassociatedwithDR.
简介:AIM:TodiscusstheimpactofLyciumBarbarumPolysaccharide(LBP)andDanshensupurifiedfromTraditionalChineseMedicine(TCM)onvascularendothelialgrowthfactor(VEGF)ofrabbitswithretinalneovascularization.METHODS:Fortyrabbitsweredividedintonormalcontrolgroup,modelcontrolgroup,LBPgroupandDanshensugroup.Animalsinthenormalcontrolgroupwerefedinthenormaloxygenenvironment.Animalsintheotherthreegroupswereputintotheenvironmentwith70%oxygenfor5daysinordertobuildthemodelofoxygen-inducedvascularproliferationretinopathy.AndthendifferentTCMextractwasinjectedintotheabdominalcavitiesoftheseannimals.After7days,theVEGFcontentofintheserumofrabbitwasmeasuredbydoubleantibodysandwichmethod.RESULTS:DataanalysisindicatedthatVEGFcontentwasasfollows:Danshensugroupwaslowerthanmodelcontrolgroup(12.92±3.84ng/Lvs19.32±4.15ng/L,P<0.05);LBPgroupandnormalcontrolgroupwerelowerthanmodelcontrolgroup(12.92±3.84ng/L,9.26±1.61ng/Lvs19.32±4.15ng/L,P<0.01);totalbloodviscosity,plasmaviscosity,cholesterolcontent,fibrinogencontentandtriacylglycerolcontentafterperitonealinjectionofLBPandDanshensuwereobviouslylowerthanbeforeinjection.CONCLUSION:TCMextract-LBPandDanshensucanprominentlyreducethecontentofVEGFintheprocessofvascularproliferativeretinopathyofrabbit;canpreventtheoccurrenceofretinalmicrovasculardiseasebyimprovingpartialoxygen-deficientenvironmentoraffectingallkindsofnewgrowthfactor.
简介:AIM:Todeterminewhethersinglenucleotidepolymorphism(SNP)rs641153isassociatedwiththeriskofage-relatedmaculardegeneration(AMD),weperformedasystematicmeta-analysisof15eligiblestudies.SNPinthecomplementfactorB(CFB)geneisconsideredtohavesignificantassociationwithAMDsusceptibility,butthereisgreatdiscrepancyintheseresults.METHODS:TheeligiblestudieswereidentifiedbysearchingthedatabasesofPubMed,EMBASE,andWebofScience.Oddsratios(ORs)with95%confidenceintervals(CIs)wereusedtoassesstheassociation.AlldatawereanalyzedusingStatasoftware.RESULTS:Theassociationbetweenrs641153andAMDriskwasstatisticallysignificantunderthehomozygousmodel(AAvsGG:OR=0.26,95%CI=0.15-0.45,P_h=0.973,/~2=0.0%,fixedeffects),dominantmodel(AA+GAvsGG:OR=0.49,95%CI=0.40-0.59,P_h=0.004,/~2=56.4%,randomeffects)andrecessivemodel(AAvsGA+GG:OR=0.30,95%CI=0.17-0.51,R_n=0.983,I~2=0.0%,fixedeffects).Thesameresultswerealsoobservedinthestratifiedanalysesbyethnicity,sourceofcontrolandsamplesize.CONCLUSION:Ourmeta-analysissuggeststhatrs641153intheCFBgenemayplayaprotectiveroleinAMDsusceptibility,thelateAMDinparticular,bothinCaucasiansandinAsians.
简介:目的:探讨胎牛血清对小鼠角膜上皮细胞生长和分化的影响。方法:分别在无血清低钙培养基(KSFM)和含100mL/L胎牛血清的KSFM中培养小鼠角膜上皮细胞。比较两种培养基中角膜上皮细胞的群体倍增(PD)。通过RT-PCR和Westernblotting方法检测p63、角蛋白19以及involucrin的表达。结果:在KSFM中,小鼠角膜上皮细胞可稳定传代超过25代,但在含胎牛血清的培养基中,细胞仅能存活3代。在KSFM中,培养细胞呈典型铺路石样外观,RT-PCR和Westernblotting结果显示细胞表达p63、角蛋白19以及involucrin。当培养基中加入胎牛血清后,细胞形态明显增大,呈扁平状;involucrin表达显著增强。结论:胎牛血清可抑制小鼠角膜上皮细胞增生、诱导其分化。
简介:目的:对影响糖尿病患者视网膜血管形态变化的相关因素进行分析。方法:选取2012-01/2016-09期间于本院就诊的2型糖尿病患者312例为研究对象。对患者进行、眼底摄片及相关实验室检查。以患者年龄、性别、病程、是否吸烟、饮酒,是否合并高血压、高血脂或糖尿病肾病等作为分组因素,比较不同组间患者出现视网膜血管形态变化的发生率,将有意义的因素再次引入Logistic回归方程,筛选出糖尿病患者视网膜血管形态变化的独立危险因素。结果:所选的312例2型糖尿病患者中,伴视网膜血管形态异常组169例,不伴视网膜血管形态异常组143例。单因素分析结果显示,年龄、病程、合并高血压、高血脂或糖尿病肾病与视网膜血管形态变化显著相关(P〈0.05),性别、是否吸烟、饮酒等与视网膜血管形态异常的发生无显著相关性(P〉0.05)。将发生视网膜血管形态异常作为因变量,上述具有统计学意义的分组因素作为自变量,经Logistic逐步回归分析结果显示,病程、患者合并高血压或糖尿病肾病是发生视网膜血管形态异常的独立危险因素(P〈0.05)。结论:病程增加、患者合并高血压或糖尿病肾病是糖尿病患者发生视网膜血管形态变化的独立危险因素。早期诊断与干预,采取措施控制血压稳定,减少肾脏损害可降低视网膜血管病变的发生率,同时减少糖尿病所引发的大血管病变,减少心脑血管不良事件的发生。
简介:AIM:Tocomparethetrabecularmeshwork(TM)andirisapoptosisoftreatedanduntreatedprimaryopenangleglaucoma(POAG)patients.METHODS:Eighttreatment-naive,newlydiagnosed(group1)and11medlcaiytreated(group2)patientswithPOAGwereincludedinthestudy.Eachpatientunderwentalimbus-basedtrabeculectomy.TheTMandperipheralirisspecimensweredissectedoutandweresnap-frozeninliquidnitrogenandstoredat-80tuntiltheywereassayed.ApoptosisineachgroupwasassesedbyTUNELmethod.RESULTS:Themeanpatientagewas60.6±5.8years(53-68years)vs58.9±8.9years(47-70years)ingroup1andgroup2(P=0.859).Themeantreatmenttimeingroup2was22.2±7.3months(12-34months).ApoptoticindexesinTMandirisweresignificantlyhigherinPOAGpatientsusingmedication(group2)comparedtotreatment-naivePOAGpatients(group1)(P=0.004,0.015;respectively).CONCLUSION:LongtermadministrationoftopicalantiglaucomamedicationscausesadditionaltoxiceffectsonTM.
简介:<正>DirSir,IamdoctorGuo-YuanYang,fromtheDepartmentofOphthalmologyofWestChinaHospitalofSichuanUniversityinChengdu,SichuanProvince,China.Iwritetopresentacasereportofposteriorlentiglobuswithcataract.
简介:目的:分析丝裂霉素C对青光眼小梁切除术后眼内压和眼血流的影响,探讨其有效性和安全性。方法:将103例青光眼患者随机分为两组,均给予青光眼小梁切除术,观察组在术中行丝裂霉素C抗瘢痕治疗;术后完成6-12mo随访,对比两组患者的临床眼压控制疗效、并发症发生率及视网膜中央动脉血流动力学指标。结果:观察组临床疗效优于对照组(P〈0.05);两组患者的总并发症发生率无统计学差异(P〉0.05),但并发症类型有所区别,观察组多发生低眼压和畏光症状,对照组多发生瘢痕性阻塞。治疗前,两组患者的眼压动力学指标无统计学差异(P〉0.05);治疗后1mo,观察组收缩期峰速(peaksystolicvelocity,PSV)与舒张末期血流速度(enddiastolicvelocity,EDV)明显低于对照组,阻力指数(resistanceindex,RI)与搏动指数(pulsatilityindex,PI)明显高于对照组,差异有统计学意义(P〈0.05);治疗后6mo,观察组PSV与EDV明显高于对照组,RI与PI明显低于对照组,差异有统计学意义(P〈0.05)。结论:丝裂霉素C对青光眼小梁切除术患者的术后不良影响时间较短,其长期疗效安全、可靠。
简介:AIM:ToinvestigatetheexpressionsoftypeIcollagen,α2integrinandβ1integrinintheposteriorscleraofguineapigswithdefocusmyopiaandwhetherbasicfibroblastgrowthfactor(bFGF)injectioninhibitstheformationanddevelopmentofmyopiabyupregulatingtheexpressionoftypeIcollagen,α2integrinandβ1integrin.METHODS:After14daysoftreatment,therefractivestateandaxiallengthweremeasuredandthelevelsoftypeIcollagen,α2integrinandβ1integrinwereassayedintheposteriorscleraeofgroupsofguineapigsthatworeamonocular-7Dpolymethylmethacrylate(PMMA)lensorhad-7DlenswearfollowedbytheperibulbarinjectionofPhosphateBufferSolution(PBS)orbFGF.Theuntreatedfelloweyeservedasacontrol.Guineapigswithnotreatmentservedasnormalgroup.·RESULTS:Theresultsshowedthat14daysofmonoculardefocusincreasedaxialeyelengthandrefraction,whilebFGFdeliveryinhibitedthemmarkedly.Further,itwasalsofoundthatthemonocular-7DlenscoulddecreasethelevelsoftypeIcollagen,α2integrinandβ1integrinexpressions,while,unlikePBS,bFGFincreasedthemsignificantlyincomparisontocontralateralcontroleyesandnormaleyes.CONCLUSION:bFGFcanpreventtheformationanddevelopmentofdefocusmyopiabyupregulatingtheexpressionsoftypeIcollagen,α2integrinandβ1integrin.Takentogether,ourresultsdemonstratethatbFGFpromotesscleraremodelingtopreventmyopiainguineapigs.
简介:目的探讨电话回访、微信等方式对闭角型青光眼患者出院后进行延伸护理服务,以提高患者遵医行为和改善护患关系的影响。方法将160例闭角型青光眼患者随机分为对照组和观察组各80例,对观察组患者于出院后第一周开始电话回访,以后分别于两周、一月、三月、六月、一年回访。对照组采用常规方法。一年后对2组患者遵医行为和再入院人数及满意度进行评价。结果观察组患者在随访期间遵医行为人数及满意度调查,明显好于对照组,再入院人数明显少于对照组(P〈0.01)。结论延伸护理服务有利于患者掌握闭角型青光眼相关的健康教育知识,增强患者遵医行为,调动患者的主观能动性,对延缓病情进展、预防并发症、减少再入院次数、减轻经济负担、提高生活质量起到了促进作用。
简介:目的:研究血管紧张素Ⅱ受体拮抗剂坎地沙坦对糖尿病(DM)大鼠视网膜组织VEGF和MCP-1表达的影响.方法:链脲佐菌素(STZ)制备DM大鼠动物模型36只,随机分为DM模型组和坎地沙坦治疗组,另取18只正常SD大鼠作为正常对照组,每组均随机分为4,8,12wk3个亚组.视网膜铺片联合PAS染色观察视网膜微血管形态学变化,应用SABC免疫组织化学法检测坎地沙坦对大鼠视网膜组织VEGF和MCP-1表达的影响.结果:正常对照组视网膜血管网结构清晰,走行规则;DM模型组血管迂曲阻塞,走行不规则;治疗组见血管网迂曲情况较模型组明显改善,走行较规则.在对照组和模型4wk组中大鼠视网膜组织无VEGF和MCP-1阳性表达或只呈弱阳性表达;模型8wk和12wk组两者阳性表达明显增强,且随着病程延长呈递增趋势.治疗组两者的表达则均较同时期模型组明显减弱,差异有统计学意义(P〈0.05).结论:研究血管紧张素Ⅱ受体拮抗剂坎地沙坦可降低DM大鼠视网膜VEGF和MCP-1的表达.