简介：Objective:Toestablishananimalmodeloflike-auditoryneuropathyinneonatalrat.MethodsTheani-malswereinjectedwithphenylhydrazinehydrochlorideorsalineat7-dayofage.ABRandDPOAEwereperformedtoassesstheauditoryfunction.Thecochleabasilarmembranestretchedpreparationandcochlearfrozensectionswerepreparedforimmunohistochemicalstainingtoexaminethemorphologicalchangeofhaircellsandspiralganglioncells(SGNs).ResultsAt7-dayagetheABRwaveI,III,V,latenciesandI-III,I-VIWIsintheexperimentalgroupweresignificantlyprolongedcomparedwiththoseinthecontrolgroup.TheABRthresholdswerealsoelevatedintheexperimentalgroup.Wefoundthereisnosignificantdiffer-enceinDPOAEinphenylhydrazinehydrochlorideexposuregroupcomparetocontrolgroup.Thecochlearhaircellsshowednosignsoflossinbothgroup,butthetotalnumberofneurofilamentspositivecellsinSGNsweresignificantlyreducedinthephenylhydrazinetreatedanimals.ConclusionOurstudysuggeststhatphenylhydrazinehydrochloridecanchangetheauditoryfunctionandinduceperipheralnervepathologybytargetedmainlytheSGNsinneonatalrat.

简介：ObjectiveAdipose-derivedstemcells(ADSCs)aresuggestedtopossessahighlyplasticabilitytodifferentiateintoseveralspecificcelltypesinadditiontoadipocytelineages,includinggermlayertissue-specificcelllineagessuchaschondrocyte,myocyte,neuronal,andosteoblastlineages.TheaimofthisstudyistoestablishaninvitroculturetechniqueforADSCsinanadultguineapigmodelthatfacilitatetheirdifferentiationintohaircell-likecells.MaterialsandMethodsCellsfrominguinalfatpadsinadultguineapigswereculturedwithβ-mercaptoethanol,RA,Forskorin,Heregulin,bFGF,BDNFandEGF.Cellulardifferentiationwasexaminedusingimmunocytochemistrytechniques.ResultsTheADSCsdemonstratedhaircellimmunophenotypeswithexpressionofepitopesofthehaircellmarkerproteinmyosinⅦa.ConclusionADSCsfromadultguineapigadiposetissuecandifferentiateintohaircell-likecellswhenculturedinvitro.ADSCsmayserveasseedcellsfortissueengineering.