FractionsofplasmaproteinofmaleKunmingmice(bodyweight24.2±0.3g)，treatedwithCisplatini.p.injectionindoseof10mg/kg,wereobtainedbyseparationonSephadex-G-50columns,bufferedwithammoniumacetatetopH5.7,TheXSRFexperimentswereperformedattheBEPC(BeijingElectronPositronCollider)synchrotronradiationfacility.Theelements(Pt,S,Ca,Fe,Ni,Cu,Zn,Se,BrandSr)inthefractionoftheplasmaproteins(>22KD)wereassayedusinghighlysensitiveSXRF.TherelativeconcentrationsofelementswerecalculatedbyanormalizationofCOmptonscatteringintensityaround22KeV,afterthenormalizationforcollectingtimeofX-rayspectrumandthecountingoftheionchamber,andsubractingthecontributionofthepolycarbonatefilmusedforsupportingthesamples.ThedeterminationcouldprovethattheelementPtinplasmawasboundwithmacro-molecularprotein.CuandSwerepresentinthefractionoftheproteininmicetreatedwithCisplatinandexhibitedanincrease,therationoftreated/controlwere1.66±0.06and1.78±0.33repectively,whereasZndecreasedtoaratioof0.78±0.09,OurresultsareinagreementwithotherswhichshowedthatCisplatinexposureleadstoamarkedlossofkidneycopper,andamoderateriseindidneyzinc.However,thisworkmainlyfocussedontheimplementationofthisanalyticalprocedure,butnotontheresultsoftheinvestigationsoftheeffectofCisplatinontraceelementsinplasmaprotein.
