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  • 简介:BaosteelTechnicalResearch,aquarterlyjournal,whichisissueddomesticallyandabroad,isrunandsponsoredbyBaosteelGroupCorporation.BaosteelTechnicalResearchmainlyreportstheachievementsintechnologicalinnovation,academicresearch,newproductdevelopmentandindustrialequipmentimprovementbyBaosteel.Itwillcontinuetofollowup

  • 标签: 宝钢集团 技术 科技创新 学术研究
  • 简介:ObjectToexploretheproceduresinper-operativeharvestingandmanagementoffreshhumancochlearspecimensforresearch.MethodsDuringtrans-cochlearsurgerytoremovelargepetro-clivalmeningiomascausinglife-threateningcompressiononthebrainstem,cochleaearenormallydestroyedanddrilledawayinordertoreachtheapicalpetrousandclivusregion.Insteadthecochleacanbedissectedoutafterethicalper-missionwasobtainedfromthelocalethicalcommittee(EPN)andallowancegainedfromthepatients.Sur-geryisperformedbyateamconsistingofoto-andneurosurgeonsasatwo-dayprocedurewithtotalpetro-sectomyincombinationwithaninferiorre-routingofthefacialnerve.Fixationofthecochleaewasdoneintheoperatingroomassoonasthespecimenshadbeenseparatedfromthetemporalbones.Decalcificationbeganafterhours’toovernight’sfixationfor4weeks.Sectioningparalleltothemodiolus(mid-modiolus)wasperformedwithacryostatmicrotome.Thesectionsweresubjectedtoimmunofluorescence(IF).ResultsUsingfreshlyprepared4%paraformaldehyde(PFD)solution,adequatefixationoffineinnerearstructureswasachievedwithhours’immersionofthecochlearspecimens.Decalcificationin6.2%ethylenedi-amine-tetraceticacid(EDTA)solutionfor4weeksyieldedathoroughlydecalcifiedcochlea.Experiencesinprocessing14humancochleaeandanalysingmainlandmarksinfivehumaninnerearplastic/siliconecastsshowedthattheovalwindow/stapesfootplatearebackwardtilted,atanangleabout15degrees,fromtheplaneperpendiculartothemodiolaraxis.Thedistancefromthemodiolarapextotheanteriorborderoftheovalwindow/footplateintheseinnerearcastsmeasuredbetween4and5mm.HighqualityIFstainingwasobtained.ConclusionSurgicallyobtainedhumancochlearspecimen,whenproperlyprocessed,containside-allypreservedantigenicityforimmunohistochemicalstudy.Adequateorientationduringsectioninghelpsob-tainoptimalmid-modiolarsectionsshowingfi

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