简介：TagetonesA(1)andB(2),twonewmonocyclicditerpenoidswereisolatedfromthen-hexanefractionoffreshflowersofTagetesminutaL.(Asteraceae).Theirstructureswereestablishedbymultiplespectroscopicmethods(IR,HR-ESI-MS,and1D-,and2D-NMR),inadditiontocomparisonwithliteraturedata.Compound1showedcytotoxicactivitytowardsMCF7andA549cancercellswithIC_(50)valuesbeing4.68and4.24μmol·L~(-1),respectively,comparedtodoxorubicin(IC_(50)0.13and1.12μmol·L~(-1),respectively).Compound2alsoexhibitedsignificantactivityagainstHCT116cancercells(IC_(50),6.30μmol·L~(-1)).

简介：Apotexperimentwascarriedouttostudytheeffectsofthreearbuscularmycorrhizalfungi(AMF),includingGlomusintraradices,GlomusconstrictumandGlomusmosseae,onthegrowth,rootcolonizationandCdaccumulationofmarigold(TageteserectaL.)atCdadditionlevelsof0,5and50mgkg-1insoil.Thephysiologicalcharacteristics,suchaschlorophyllcontent,solublesugarcontent,solubleproteincontentandantioxidantenzymeactivity,ofTageteserectaL.werealsoinvestigated.ThesymbioticrelationshipbetweenthemarigoldplantandarbuscularmycorrhizalfungiwaswellestablishedunderCdstress.ThesymbioticrelationshipwasreffectedbythebetterphysiobiochemicalparametersofthemarigoldplantsinoculatedwiththethreeAMFisolateswherethecolonizationratesintherootswerebetween34.3%and88.8%.Comparedwiththenon-inoculatedmarigoldplants,theshootandrootbiomassoftheinoculatedmarigoldplantsincreasedby15.2%-47.5%and47.8%-130.1%,respectively,andtheCdconcentrationandaccumulationdecreased.ThechlorophyllandsolublesugarcontentsinthemycorrhizalmarigoldplantsincreasedwithCdaddition,indicatingthatAMFinoculationhelpedthemarigoldplantstogrowbyresistingCdstress.TheantioxidantenzymesreacteddifferentlywiththethreeAMFunderCdstress.ForplantsinoculatedwithG.constrictumandG.mosseae,theactivitiesofsuperoxidedismutase(SOD)andcatalase(CAT)increasedwithincreasingCdaddition,butperoxidase(POD)activitydecreasedwithincreasingCdaddition.ForplantsinoculatedwithG.intraradices,threeoftheantioxidantenzymeactivitiesweresignificantlydecreasedathighlevelsofCdaddition.Overall,theactivitiesofthethreeantioxidantenzymesintheplantsinoculatedwithAMFwerehigherthanthoseoftheplantswithoutAMFinoculationunderCdstress.Ourresultssupporttheviewthatantioxidantenzymeshaveagreatinfluenceonthebiomassofplants,andAMFcanimprovethecapabilityofreactiveoxygenspecie