简介:Defenceproteinsareakindofchemicaldefencecompounds.Theyplayakeyroleinplantrestrainingbioticandabioticharm.Toilluminateactivitiesofsomedefenseproteinsassociatedwithageorplantfamilyoflarch,thelarchneedleswerecollectedfromtwodifferentfamiliesofKoreanlarch,Larixolgensisandahybridlarch,L.kaempferi×L.gmelinii,respectively,andthentheactivitiesofdefenceproteinsinthoseweretestedusingaUVspectrophotometry.Theresultsshowedthattheactivitiesofprotectiveenzymesatthe16-/17-and19-year-agegroupswerehigherthanthoseattheotheragegroupsinthebothlarchspecies.Whiletheactivitiesofpolyphenoloxidase(PPO)atthe16-/17-year-agegroupandphenylalanineammonia-lyase(PAL)andproteaseinhibitorsatthe19-year-agegroupwerethehighestamongallagegroups.Then,comparedwithL.olgensis,thehybridlarchhadsignificanteffectsontheactivitiesofprotectiveenzymes.TheeffectsofplantfamilyontheactivitiesofPALandchymotrypsininhibitorweresignificantlydifferent,andthenthoseontheactivitiesofPPOwerenotsignificantlydifferent.TheeffectsofthetwofamiliesinL.olgensisontheactivitiesoftrypsininhibitor(TI)weresignificantlydifferent,whilethoseinthehybridlarchontheactivitiesofTIwerecontrary.Toconclusionsshowedthatthedifferentageandplantfamilysignificantlyaffectedtheactivitiesofdefenceproteinsintheneedlesoftwolarchspecies,andthenenhancedthelarchresistancetopests.Thesecouldplayakeyfunctioninforestrytreegeneticimprovementandmanagementinfuture.
简介:将初始体质量(0.42±0.05)g的杂交鲟(Acipenserschrenckii♀×A.baerii♂)仔鱼饲养在室内循环水养殖系统中,投喂添加0(G1)、0.25%(G2)、0.5%(G3)、0.75%(G4)和1.0%(G5)L-丙氨酰-L-谷氨酰胺(L-AG)的5种等氮饲料,每个处理3个重复,每个重复1500尾鱼,研究L-AG水平对杂交鲟体成分和十二指肠形态的影响。56d的养殖结果表明:饲料中添加0.25%和5.0%L-AG对全鱼粗蛋白含量影响不显著(P〉0.05),但添加0.75%和1.0%L-AG显著提高了全鱼粗蛋白含量(P〈0.05)。添加0.25%~1.0%L-AG对全鱼水分、粗脂肪和灰分含量影响不显著(P〉0.05)。与对照组相比,添加0.25%~1.0%L-AG对肠道绒毛高度、微绒毛高度和肌层厚度均未产生显著影响(P〉0.05)。结果表明:外源补充0.75%~1.0%L-AG可显著提高杂交鲟仔鱼粗蛋白含量,但添加0.25%~1.0%L-AG对十二指肠形态不产生显著影响。
简介:MultiplicationofElaeagnusangustifoliaL.wasexaminedinvitrosuccessivelyfromasingleshootunderthespecifiedconditionofdifferentmedia,plantgrowthregulators,pHvalueandsucroseconcentration.ItwasshowedthatMMS1wasthemostsuitablemediumonshootmultiplicationamong5mediaconcerned;BAPwasthemosteffectiveoneamongallthecytokinininvolved,BAP,KN,TDZandZT;theexpluntofthetophalf-partfromashootproducedmorenewshootsthanthatofthefoothalf-partdid;morenewshoots(>2cm)wereproducedunder3%sucrosebetweentheconcentrationswithtophalf-partexplants;shootscouldgrowwellbetweenpH4.4andpH7.0,andthebiggestnumberofshootswasproducedinpH5.6,whileinpH5.8themaximumrootingrateappeared.Asaresult,thecombinationof0.5μMBAPand0.1μMIBAonMMS1mediuminducedthemaximumshootmultiplication.Thenumberofshootamplified3timesin1month,and3^12shoots(>2cm)mightbetheoreticallymultipliedannuallyfromasingleshoot.
简介:Twopotexperimentswithacompletelyrandomdesignand4replicationswereperformedinagreenhousetoexaminetheresponsedifferenceof17cultivarsofpakchoi(BrassicachinensisL.)growninaCu-spikedandacleansoiltoCu.relativelytolerant.Whenthe17cultivarsofpakchoigrewincleangardensoil,theCuconcentrationsintheabovegroundpartwerepositivelycorrelated(r=0.6693)withtheirrootCuconcentrations.However,whentheygrewintheCu-spikedsoilahighlynegativecorrelationcoefficient(r=-0.5376)wasobtainedintheCuconcentrationbetweentheabovegroundpartandtheroot.ThismeantthattheCutolerantcultivarshadaweakabilitytotransferCufromtheirroottotheirabovegroundpart,andthereforestoredmuchmoreCuintheirrootthantheCusensitivecultivars.