简介:Membersoftheactivityofbc1complex(ABC1)familyareproteinkinasesthatarewidelyfoundinprokaryotesandeukaryotes.PreviousstudiesshowedthatseveralplantABC1genesparticipatedintheabioticstressresponse.Here,wepresentthesystematicidentificationofriceandArabidopsisABC1genesandtheexpressionanalysisofriceABC1genes.Atotalof15and17ABC1genesfromthericeandArabidopsisgenomes,respectively,wereidentifiedusingabioinformaticsapproach.Phylogeneticanalyseso...
简介:Homeoboxtranscriptionfactorsparticipateinthegrowthanddevelopmentofplantsbyregulatingcelldifferentiation,morphogenesisandenvironmentalsignalresponse.Torevealthefunctionsofthesetranscriptionfactorsinrice,weconstructedtheRNAivectorsofOsHox9,amemberofhomeoboxfamily,andanalyzedthefunctionofOsHox9usingreversegenetics.TheplantheightandtilleringnumberofRNAitransgenicplantsdecreasedcomparedwiththoseofwild-typeplants.Reversetranscription-polymerasechainreactionanalysisshowedthatOsHox9expressionreducedinthetransgenicplantswithphenotypicvariance,whereasthatinthetransgenicplantswithoutphenotypicvariancewassimilartothatinthewild-typeplants.ThisresultsuggeststhatthephenotypesofthetransgenicplantswerecausedbyRNAieffects.Thetissue-specificityofOsHox9expressionindicatedthatitwasexpressedindifferentorgans,withhighexpressioninstemapicalmeristemandyoungpanicles.SubcellularlocationofOsHox9demonstratedthatitwaslocalizedonthecellmembrane.