简介:Objective:Toinvestigatethepost-transcriptionalregulationofp21WAF1/CIP1byp53.Methods:TheMDA-MB-468cellshaveendogenousmutantp53andtheMCF7cellslineshavewtp53.Recombinantp53expressionandp21WAF1/CIP1inductionweredetectedbyWesternblotanalysis.Northernblotanalysiswascarriedouttoexaminewhetherchangesinp21WAF1/CIP1proteinlevelsinMCF7cellstreatedwithAdCMVp53arereflectedatthemRNAlevel.FlowcytometricanalysisofMCF7cellsfollowingoverexpressionofrecombination.Results:Theratioofp53:p21WAF1/CIP1wasbelow1attheearlystagesofAdCMVp53infection,butincreasedto1.6byday3andto9.7byday5post-infection.Asexpected,p21WAF1/CIP1expressionwasnotdetectableinMDA-MB-468cellsdespitethepresenceofhighlevelsofmutantp53protein.TheG1/SratiosinuntreatedcontrolsandAdCMVβgalinfectedMCF7cellswere1.10and1.35,respectively.ByNorthernblotanalyzingthep21WAF1/CIP1:GAPDHratiosatdifferenttimepointsagainsttheratioattimepoint0,amaximum3-foldinductionofp21WAF1/CIP1mRNAexpressionrelativetountreatedcontrolwasobservedonday1post-infection.TheflowcytometricanalysisindicatedthatMCF7cellsinfectedwithAdCMVp53undergoG1arrestatbothtimepointsstudied,withG1/Sratiosrangingfrom5.54atday1to5.65atday7.TheG1/SratiosinuntreatedcontrolsandAdCMVβgalinfectedMCF7cellswere1.10and1.35,respectively.Conclusion:Thisstudydemonstratedthatp53couldregulatep21WAF1/CIP1geneexpressionatboththetranscriptionalandpost-transcriptionallevelsinMCF7cells.Thelattermechanismmaybeinvolvedinorberesponsiblefor,theinductionofcellcyclearrestbytranscription-defectivemutantsofp53.
简介:妊娠合并脑瘤后,由于早孕反应和晚期妊娠中毒,出现头痛、恶心、呕吐等症状,易与脑瘤所致之颅内压增高的症状相混淆,延误诊治。一28岁女性患者,因停经8月,头痛、恶心、呕吐,右上、下肢无力半月,于2003年6月7日入我院。检查:神志清楚,右侧上、下肢肌力Ⅲ级,肌张力增高,右侧Babinski征阳性。腹围91cm,宫高31cm,头先露,浮,胎心144次/分。MRI检查:左额叶4×3.5×5cm分叶状占位病变。T1等、低、混杂信号,T2高、等、混杂信号。周围脑白质水肿,灰质受压,左侧脑室变窄,中线结构右移。诊断:右额叶胶质瘤,妊娠8月。2003年6月17日手术,先在腰麻下行剖腹产,娩出2250克重男婴,成活良好。随即
简介:GFAPisaspecificantigenofglialelement,butAlpha-1-antichymotrypsinhasnotbeenreportedintheliterature.Alpha-1-antichymotrypsinwasguidedbyGFAPusingPAPmethodtotheastrocytesof137gliomas.120(87%)gliomaswerepositiveforAlpha-1-antichymotrypsin.Ofthese120gliomas,86(72%)gavediffusedistribution,17(14%)gavefocaldistribution,and17(14%)gavescattereddistributions.Alpha-1-antichymotrypsiningliomatissuemaybeanimportanttumormarkerfordiagnosis.
简介:患者女,36岁,因右眼球突出半月、疼痛、视物不清3天,于1996年7月21日入院.既往体健.眼部检查:视力,右0.1,左1.0;右上睑红肿,皮肤张力大;右眼球突出、向颞下方移位,眼球向内、向上运动受限;右眼球突出度为20(?)12mm;右眼鼻侧眶上缘可触及3cm×2.5cm肿物,活动度小,有压痛;右球结膜充血水肿,突出于睑缘外;眼底检查未见异常.左眼未见异常.实验检查:WBC8.8×10~9/L,N0.85、L0.15.ESR45mm/h.眼眶X线片未见骨质破坏,肿物针吸术涂片检查,找到恶性肿瘤细胞.于1996年8月5日行右眼眶内容物剜出术,术中见筛骨破坏,筛窦内充满牙膏样物,同时行筛窦根治术.肿物病理检查:大小3cm×3cm×2.5cm,无明显包膜,切面呈鱼肉样,中心部少量腐烂;镜下见大量弥漫分布的瘤细胞,形态各异,呈圆形、梭形、带状