简介：TheelectrochemicalbehaviorsandthedetectionofNO2-haveattractedincreasingattentionrecentlybecauseoftheimportantrolesplayedbyNO2-inenvironmentandlifeprocesses.Thus,advancedtechniquesforthedetermination,especiallyon-sitedetermination,ofthenitriteinfoods...

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简介：在这个工作，新奇bisbenzimidazolylpyridine-functionalized荧光灯环氧基树脂树脂为organophosphate杀虫剂被综合察觉。环氧基树脂树脂被Fourier变换红外线的光谱学(英尺红外)描绘，质子原子磁性的回声光谱学(1HNMR)，胶化浸透层析(GPC)，微分扫描热量测定(DSC)和荧光光谱学。在与Eu(III)离子装载以后，环氧基树脂树脂显示出强壮的荧光排出物。当暴露了在答案跟踪diethylchlorophosphate的数量时，荧光排放被观察即刻地被熄灭。熄灭常数K为在617 熄灭的sv；nm决心是0.377 ；× ；103 L/mol。这敏感熄灭排放的功能和聚合支持的容易的处理自然使树脂能是organophosphates的察觉的一个有希望的chemosensor候选人。

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简介：有electrochemiluminescence察觉的新、简单的毛状的电气泳动为分离和一双diastereoisomeric碱(麻黄素和pseudoephedrine)的quantification被开发。察觉的限制(S/N=3)4.5是?????????€牂????€??€午???丠？？

简介：Combiningtheinhibitedaptazymeandmolecularbeacon(MB),wedevelopedaversatilesensingstrategyforamplifieddetectionofadenosine.Inthisstrategy,theadenosineaptamerlinkstothe8-17DNAzymetoformanaptazyme.Ashortsequence,denotedasinhibitor,isdesignedtoformaduplexspanningtheaptamer–DNAzymejunction,whichblocksthecatalyticfunctionoftheDNAzyme.Onlyinthepresenceoftargetadenosine,theaptamerbindstoadenosine,thustheinhibitordissociatesfromtheaptamerportionoftheaptazymeandcannolongerformthestableduplexrequiredtoinhibitthecatalyticactivityoftheaptazyme.ThereleasedDNAzymedomainwillhybridizetotheMBandcatalyzethecleavageinthepresenceofZn2+,makingthefluorophoreseparatefromthequencherandresultinginfluorescencesignal.Theresultsshowedthatthedetectionmethodhasadynamicrangefrom10nmol/Lto1nmol/L,withadetectionlimitof10nmol/L.

简介：在microfluidic薄片的一样的地方包含同时的LIF和contactless-conductometric大小的一个联合察觉系统被描述。LIF测量根据共焦的原则被设计，一个家具contactless传导性察觉者在C4D被使用。两测量在混合物的分析互相独立、有益。影响反应的各种各样的试验性的参数被检验并且优化。表演被玫瑰精B的同时的察觉表明。并且结果证明联合察觉系统能易感知地并且可靠地被使用。

简介：Apyridoxal-basedchemosensorwassynthesizedbyreactinghydrazinehydrateandpyridoxalhydrochlorideinethanolandcharacterizedbyNMRandESI-MS.Theopticalpropertiesofthecompoundwereinvestigatedinamethanol:HEPESsolution.ThecompounddisplayedselectivityforCu2+,asevidencedbyacolorlesstoyellowcolorchange,whichwascharacterizedusingUV–visspectroscopy.ThefluorescenceofthecompoundcanbequenchedonlybyCu2+,accompanyingbyacolorchangefrombluetocolorless.Furthermore,itcanbeusedinbioimaging.

简介：Graphenenanosheets(GS)wereeasilypreparedthroughliquid-phaseexfoliationofgraphitepowderinN,N-dimethylformamide(DMF)withtheassistanceofsodiumcitrate.Then,GSwascoatedontoaglassycarbonelectrode(GCE)surfacebydroptofabricateaGS/GCEnanointerface.Subsequently,byusingtetraethylorthosilicatesolasprecursor,nanosilicawaselectrochemicallydepositedontotheGS/GCEsurfacetoproduceananocompositefilmelectrode(nanosilica/GS/GCE).Electrochemicalbehaviorsofmethylparathion(MP)onthenanosilica/GS/GCEsurfacewereinvestigatedthoroughly.Itwasfoundthatthenanosilica/GSnanocompositescanimprovetheredoxpeakcurrentsofMPsignificantlyduetothesynergeticeffect.TheoxidationpeakcurrentwaslinearlyrelatedtoMPconcentrationintherangefrom0.0005mmol/Lto5.6mmol/L.Thedetectionlimitwascalculatedtobe0.07nmol/L(S/N=3).ThedevelopedmethodwasusedtodetermineMPinrealsamples.Therecoverieswereintherangefrom95.4%to104.2%,demonstratingsatisfactoryresults.

简介：Micelles,microemulsions.vesicles,etc.,areorganicmicroheterogeneousmedia.Useofthesemediainanalyticalreactionsfordevelopingnewkinetic-baseddeterminationsorimprovingpreviouslyestablishedkineticmethodshasbeenreported'2.Inrecentyears,amixedmicellarmediumc...

简介：Fastanalysisofthiocyanatebyion-pairchromatographyusingasilica-basedmonolithiccolumnanddirectconductivitydetectionwascarriedout.ChromatographicseparationwasperformedonaChromolithSpeedRODRP-18eusingtetrabutylammoniumhydroxide(TBA)–phthalicacid–acetonitrileaseluent.Theeffectsofeluentconcentration,eluentpHvalue,columntemperatureandflowrateonretentiontimeofthiocyanatewereinvestigated.Theoptimizedchromatographicconditionsforthedeterminationofthiocyanatewereasfollows:0.25mmol/LTBA–0.18mmol/Lphthalate–7%acetonitrile(pH5.5)aseluent,columntemperatureof308C,andflowrateof6.0mL/min.Retentiontimeofthiocyanatewaslessthan1minundertheconditions.Commonanions(Clà,NO3à,SO42àandIà)didnotinterferewiththedeterminationofthiocyanate.Detectionlimit(S/N=3)ofthiocyanatewas0.96mg/L.Calibrationgraphbetweenpeakareaandtheconcentrationofthiocyanatewaslinearintherangeof2.0–100.0mg/L.Relativestandarddeviation(RSD)ofchromatographicpeakareawas1.4%(n=5).Thismethodhasbeenappliedtothedeterminationofthiocyanateinionicliquids.Recoveriesofthiocyanateafterspikingwere100.5%.

简介：基于有安培计的察觉(CZE广告)的毛状的地区电气泳动，一个简单、可靠、可再现的方法包括paracetamol，维生素C，咖啡的酸和chlorogenic酸在VcYinqiao药片为四活跃成分的同时的决心被开发了。一个碳磁盘电极被用作工作电极和0.95V(对SCE)作为察觉潜力被选择。CZE实验的最佳的条件是30mmol??Д

简介：Ahighlysensitiveelectrochemiluminescence-polymerasechainreaction(ECL-PCR)methodforK-raspointmutationdetectionisdeveloped.Briefly,K-rasoncogenewasamplifiedbyaRu(bpy)32+(TBR)-labeledforwardandabiotin-labeledreverseprimer,andfollowedbydigestionwithMvaIrestrictionenzyme,whichonlycutthewild-typeampliconcontainingitscuttingsite.Thedigestedproductwasthenadsorbedtothestreptavidin-coatedmicrobeadthroughthebiotinlabelanddetectedbyECLassay.TheexperimentresultsshowedthatthedifferentgenotypescanbeclearlydiscriminatedbyECL-PCRmethod.Itisusefulinpointmutationdetection,duetoitssensitivity,safety,andsimplicity.

简介：二新奇水溶性的indocyanine染料被综合。他们的化学结构被1HNMR，红外，MS和紫外力的技术证实。在不同溶剂的准备染料的吸收被测量。二染料展出了否定solvatochromism，与吸收和排放最大值在的蓝移动(牛的浆液白朊)protonic溶剂相对非protonic溶剂。处于免费状态和BSA的染料的荧光性质被调查。为Dye2的荧光紧张的重要改进面对BSA被观察。Dye2能作为荧光灯被建议为在水的答案的BSA察觉的染料。

简介：简单、快速、精确的高效液体色析法(HPLC)技术结合了化合光(CL)察觉为肾上腺素(E)，noradrenaline(NA)和多巴胺(DA)的同时的决心被开发。它在在发光氨和钾铁氰酸盐之间的CL反应上基于analyte改进效果。各种各样的参数的效果发光氨集中，pH价值和analytes(E，NA和DA)的色析法的行为上的活动阶段的部件例如钾铁氰酸盐集中，被调查。分离用0.01mol/L钾氢phthalate答案和甲醇的活动阶段在C18列上被执行(92:8，V/V)。在1愠?桰獹潩潬楧慣?潣摮瑩潩?眠楨档椠?的范围在最佳条件，E，NA和DA下面显示出好线性关系???鶍?????鐿?醭靠??颮題????鞪吗？？

简介：在Au的鼹鼠比率的5nmAuPdnanoalloy:Pd=32:1被准备，作为reductant把钠柠檬酸盐用作稳定的代理人和NaBH4。AuPdnanoalloy被aptamer修改准备aptamer-AuPd(AptAuPd)为回声散布的探查(RS)5.01250nmol/LHg2+的察觉。AptAuPd-Hg2+aptamer反应答案被膜过滤，并且在filtrate的AptAuPd展出了强壮的催化效果在上慢捏粒子反应在之间NiCl2和NaH2PO2，和NiP粒子在508nm显示出一座RS山峰。当Hg2+集中增加了时，RS紧张减少了。减少的RS紧张对在0.51250nmol/L的范围的Hg2+集中线性。RS试金被用来在真实样品决定Hg2+，与好结果。

简介：Serine,oneofthenonessentialaminoacids,isofprincipalinterestbecauseofitscapabilitytoformmagic-numberionicclusters,whichprovidearemarkablepreferenceforhomochirality.WithL-asparticacidastheprecursor,thisstudyprovidesexperimentalevidenceforserineformationinweakacidifiedaqueoussolutionsinthepresenceofiron,withexposuretosunlight,whichsimulatesthenaturalconditionsoftheprebioticaqueousenvironment.Theresultantmixtureisdirectlyanalyzedviadesorptionelectrosprayionizationmassspectrometry(DESI-MS),withoutanysamplepreseparation.Theserinemonomerissuccessfullydetectedasprotonatedmolecules,givingapeakatm/z106,whichisconfirmedbytheMS/MSfragments.Protonatedserineoctamer(m/z841)isalsoobservedwithsignificantabundanceintheMSspectraandisconfirmedbytheMS/MSdata,whichshowstheformationoftheserineoctamerbyasynthesizedserineintheresultantmixture.ItisalsofoundthattheserineoctameryieldedequivalentabundanceintheDESImassspectrainawidepHrange(pH=1-5),andthatexistenceofferroussaltandsunshineareessentialfortheconversionofasparticacidtoserineintheacidicwatersolution.

简介：这份报告描述了能够基于调停toehold的海滨排水量反应(toehold-SDR)和microbead俘获技术的联合在正常温度检测单个核苷酸多型性的一个免费酶、方便、便宜的genotyping生物传感器。生物传感器由一根记者探针和一根俘获探针形成的pre-hybridized海滨组成。面对一个变异的序列，没有调停toehold的海滨排水量，记者探查不能被免除pre-hybridized海滨。Microbeads俘获荧光灯通过biotin-streptavidin相互作用的pre-hybridized海滨microbeads给出重要荧光信号，当在答案没有荧光时。面对一个匹配的目标，然而，海滨排水量有效地被开始，记者探查被免除pre-hybridized海滨。在增加microbeads以后，解决方案生产明亮的荧光，当时microbeads没有明显的信号。遗传型根据答案的荧光紧张方便地被识别。方法提供简单、便宜的策略检测点变化。而且，这个生物传感器在1-40 的范围显示出线性关系；nmol/L和活动范围0.3 的察觉限制；nmol/L。

简介：Amethodusingcarboxymethyl-β-cyclodextrin(CM-β-CD)asselectorforchiralseparationofaminoacidsbycapillaryelectrophoresisandlaser-inducedfluorescencedetectionwasstudied.Resolutionwasbetterthanthatobtainedbyβ-CDorHP-β-CD.

简介：Amantadine(AMA)是在养蜂过去常保护蜜蜂免于sacbrood病毒(Moratoraetatulae)的抗病毒的药。这研究描述了可靠高效的液体色析法(HPLC)为用稳固阶段的抽取(SPE)在蜂蜜分析AMA的方法为纯化的子弹(PlexaPCX)，4-fluoro-7-nitro-2,1,3-benzoxadiazole(NBD-F)作为一个列前derivatization代理人，和fluorometric察觉(ex=470nm，em=530nm)。色析法的分离在XDBC18列上被执行(150