简介:Theobjectiveofthestudywastoelucidateopticalcharacteristicsofthechromophorestructuresoffluorescentproteins.RamanspectraofcommonlyusedGFP-likefluorescentproteins(FPs)withdiverseemissionwavelengths(green,yellow,cyanandred),includingtheenhancedhomogenousFPsEGFP,EYFP,andECFP(fromjellyfish)aswellasmNeptune(fromseaanemone)weremeasured.High-qualityRamanspectrawereobtainedandmanymarkerbandsforthechromophoreoftheFPswereidentifiedviaassignmentofRamanspectrabands.WereportthepresenceofapositivelinearcorrelationbetweentheRamanbandshiftofCs=C6andtheexcitationenergyofFPs,demonstratedbyplottingabsorptionmaxima(cm-1)againstthepositionoftheRamanbandCs=C6inEGFP,ECFP,EYFP,theanionicchromophoreandtheneutralchromophore.ThisstudyrevealednewRamanfeaturesinthechromophoresoftheobservedFPs,andmaycontributetoadeeperunderstandingoftheopticalpropertiesofFPs.