Inhibitive Determination of Heavy Metal Ions by Conductometric Nitrate Reductase Biosensor-中国期刊网

摘要 Aconductometricnitratebiosensorbasedonmethylviologen/Nafion/nitratereductase(NR)interdigitatedelectrodeforheavymetaldeterminationwasproposed.TheactivityofNR(EC1.6.6.2)fromAspergillusniger(A.niger)wasassayedasafunctionofmetalconcentrationinthepresenceofCu2+,Zn2+,Cd2+andPb2+.Parametersinfluencingtheperformanceofthebiosensorwereoptimizedfortheapplicationofitintheinhibitiondeterminationofheavymetalions.DetectionlimitsforCu2+,Zn2+,Cd2+andPb2+wereabout0.05,0.5,0.1and1.0μmol/L,respectively.TheresultsshowthatNRactivitycouldnotberegainedafterexposuretoCu2+,butcouldbepartiallyrecoveredafterexposuretoZn2+,Pb2+andCd2+.

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Inhibitive Determination of Heavy Metal Ions by Conductometric Nitrate Reductase Biosensor

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Inhibitive Determination of Heavy Metal Ions by Conductometric Nitrate Reductase Biosensor

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