摘要
Toinvestigatetheprotectiveeffectofepigallocatechingallate(EGCG)ontheimmunefunc-tionofdendriticcells(DCs)afterultravioletBirradiation(UVB)anditsunderlyingmechanisms,themonocyteswereisolatedfromperipheralbloodandcultivatedintoDCswithcytokines,suchasGM-CSFandIL-4.DCswereharvestedaftercultivationfor7dandsubjectedtoirradiationwithdifferentdosagesofUVB.Then,200μg/mlofEGCGwereaddedincertaingroupsimmediatelyafterirradiation.DCssimplytreatedwithUVBortreatedwithbothUVBandEGCGwereco-culturedwithlymphocytes,andMTFassaywasusedtodetecttheabilityofDCstostimulateproliferationoflymphocytes.SurfacemarkersCDS0,CD86,HLA-DRandCD40weredetectedbyflowcytometry,andthelevelsofIL-10andIL-12secretedfromDCs24haftercultivationweremeasuredbyELISA.ItwasdemonstratedthatUVBirradiationcouldinhibittheabilityofDCstostimulatetheproliferationoflymphocytesandsurfaceexpressionsofCDS0,CD86,HLA-DRandCD4onDCsinadose-dependentmanner.TheinhibitionrateofDCswasimprovedtosomeextentaftertreatmentwith200μg/mlofEGCG.Whentheconcentra-tionofEGCGexceeded100μg/ml,theenhancingeffectofEGCGontheexpressionoftheco-stimulat-ingmoleculesonDCscouldbedemonstratedinadose-dependentmanner.UVBshowednosignificantinfluenceonthesecretionofIL-10andIL-12fromDCs,whileEGCGcoulddown-regulatethesecretionlevelofIL-12andup-regulatethatofIL-10.ItisconcludedthatEGCGcanantagonizetheinhibitoryeffectonDCsinducedbyUVBirradiation.Thisfunctionhassomerelationshipwithitsprotectingeffectoftheexpressionoftheco-stimulatingmoleculeonthesurfaceofDCsandthesecretionlevelofIL-10andIL-12.
出版日期
2007年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
