单因素实验初步提取苦丁茶中总黄酮

总黄酮 纤维素酶 微波提取 单因素实验中图分类号 R2 文献标号 A 文章编号 2095-7165(2015)16-0140-01Optimal extracting conditions for f lavonoids in Leaves of Llex latifolia and the value of itshealth care researchWU Rui-ling(China pharmaceutical university college of pharmacy Nanjing 210000 China)Abstract ObjectiveTo obtain the optimal conditions for extacting flavonoids in Kuding tea by single-factor experiment. MethodsExtracting theflavonoids in Kuding tea using cellulose-microwave method single factor and single-factor experiments were applied to obtain the best extractiontechnology. ResultsThe optimum extraction condition was based on 0.5h cellulose hydrolysis at 45℃ and PH 5.0 with an enzyme dose of 0.02％followed by extraction with 40％ ethanol 30ml under the assistant of microwave treatment for 50s. The average yield of flavonoids is more than enzymemethod and microwave method. ConclusionThe methods can be reference to extract flavonoids in Kuding tea. It is necessary to separate and purifyextraction to get accura