摘要
Objective:Toinvestigateanewmethodtoconstructtissue-engineeringbonethatwillbeapplicableclinically. Methods: Thecultured5thgenerationrabbitbonemarrowstromaosteoblasts(MSO)wasdissolvedin3%sodiumalginatesolution(thefinalconcentrationofsodiumalginateinthesolutionbeing1%,andMSO,5×106/L),andtheninoculatedintopreparedtrueboneceramic(TBC)andgelatinizedthebonebydribblingwithcalciumgluconate.Thestandardbonedefectmodelsweremadein48adultNewZealandrabbitsbothradius.Amongthe48rabbits,24wereinGroupsAandB,inwhichtheleftradiuswasimplantedwithgelatinizedMSO-TBC(GroupA)andrightradiusimplantedwithautograft-bone(GroupB);andtheother24wereincontrolgroupwhoseleftradiuswasimplantedwithnon-gelatinizedMSO-TBC(GroupC)andrightradiusimplantedwithgelatinizedTBC(GroupD).Outcomesoftheimplantedboneswereassessedbyradiology,pathologicalhistology,osteogeneticquantitativeanalysis,andbiomechanicsat2,4,8,12weekspostoperatively.Results: InGroupsAandB,asatisfactorybonereparationandbonyunionwasnotedwithin12weeks.InGroupsCandD,bonereparationwasnotsatisfiedcomparedwithGroupAintermsofostogeneticquantityandbiomechanics. Conclusions: GelatinizedMSO-TBCisanidealartificialactivebonethatovercomesTBCshortcomingsoffragilenessandsmoothsurfacethatisnoteligibleforseedcellsadhesion.Itispromisingtoputintoclinicaluseextensively.
出版日期
2005年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
