摘要
Diversityandcompositionoflepidopteranmicrobiotasarepoorlyinvestigated,especiallyacrossthedifferentdevelopmentalstages.Toimprovethisknowledge,wecharacterizethemicrobiotaamongdifferentdevelopmentalstagesoftheIndianmealmoth,Plodiainterpunctella,whichisconsideredoneofthemajorpestofcommoditiesworld-wide.Usingculture-independentapproachbasedonIllumina16SrRNAgenesequencingwecharacterizedthemicrobiotaoffourdevelopmentalstages:eggs,first-,andlast-instarlarvae,andadult.Atotalof1022bacterialOTUswereobtained,showingaquitediversifiedmicrobiotaassociatedtoalltheanalyzedstages.ThemicrobiotasassociatedwithP.interpunctellaresultedalmostconstantthroughoutthedevelopmentalstages,withapproximately77%ofbacterialOTUsbelongingtothephylumofProteobacteria.ThedominantbacterialgenusisrepresentedbyBurkholderia(?64%),followedbyPropionibacterium,Delftia,Pseudomonas,andStenotrophomonas.Acorebacterialcommunity,composedof139OTUs,wasdetectedinallthedevelopmentalstages,amongwhich112OTUswereassignedtothegenusBurkholderia.Aphylogeneticreconstruction,basedonthe16SrRNA,revealedthatourBurkholderiaOTUsclusteredwithBurkholderiacepaciacomplex,inthesamegroupofthoseisolatedfromthehemipteransGossypariaspuriaandAcanthococcusaceris.Thefunctionalprofiling,predictedonthebaseofthebacterial16SrRNA,indicatesdifferencesinthemetabolicpathwaysrelatedtometabolismofaminoacidsbetweenpreimaginalandadultstages.Wecanhypothesizethatbacteriamaysupporttheinsecthostduringpreimaginalstages.
出版日期
2019年03月13日（中国期刊网平台首次上网日期，不代表论文的发表时间）
