Optimization for Microbial Degradation of Dibenzothiophene by Pseudomonas sp. LKY-5 Using Response Surface Methodology-中国期刊网

摘要 Inthisresearch,thedegradationofdibenzothiophene(DBT)wasinvestigatedbyusingPseudomonassp.LKY-5isolatedfromoilcontaminatedsoil.Theresponsesurfacemethodology(RSM)basedontheBox-Behnkendesign(BBD)wasappliedforevaluatingtheinteractiveeffectsoffourindependentvariablesincludingsubstrateconcentration,temperature,pHandagitationrateontheDBTremovalresponse.Atotalof29experimentsforfourfactorsatthreelevelswereconductedinpresentstudy.Asecond-orderregressionmodelwasthendeveloped,andtheanalysisofvariance(ANOVA)illustratedthattheproposedquadraticmodelcouldbeutilizedtonavigatethedesignspace.Thevalueofdeterminationcoefficient(R2=0.9534)indicatedasatisfactoryagreementbetweenthequadraticmodelandtheexperimentaldata.ItwasfoundthatDBTremovalwasmoresignificantlyaffected(P<0.0001)bysubstrateconcentrationcomparedwithotherthreeparameters.An100%degradationofDBTcouldbeobtainedbyPseudomonassp.LKY-5atasubstrateconcentrationof100mg/L.

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Optimization for Microbial Degradation of Dibenzothiophene by Pseudomonas sp. LKY-5 Using Response Surface Methodology

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Optimization for Microbial Degradation of Dibenzothiophene by Pseudomonas sp. LKY-5 Using Response Surface Methodology

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